Gel Filtration

Experiment 1-7 Lab report

change Filtration Chromatography:
Desalting of a protein containing resoluteness

Group D:
Viktorija Glembockyte and Laura Panavaite
v.glembockyte@jacobs-university.de and l.panavaite@jacobs-university.de

GRADE Jacobs University Bremen

SIGNATURE 15/12/08

Experiment 1-7. Gel Filtration Chromatography: Desalting of a protein containing theme

Introduction
The biochemical characterization of an individual compound is all-important(a) for the understanding of this molecule’s function within the cell. such(prenominal) characterization can be performed only after polish of respective compound. Different properties of a biomolecule can be employ for the breakup. Proteins are usually separated using chromatographic techniques. In this experiment we used gel filtration chromatography – method based separation of molecules according to their size. Using this technique, BSA (Bovine Serum Albumin) was separated from a solution containing it. Two different spectroscopic assays were carried out: the Bradford Assay and protein stopping point by absorbance measurement at 280 nm. These enabled us to identify and mensurate the BSA containing samples gained using gel chromatography separation technique (Illenberger et al., 2008).





Materials and Methods
The experiments were carried out as described in the manual with the following exceptions: in the photometrical analysis of gel fractions at 280 nm both samples were weaken twice to obtain safe absorption values smaller than 1 for higher accuracy. When measuring BSA absorbencies for the calibration curve, all standards were lively twice, not once, therefore a mean value of cardinal different samples was used for obtaining each point of the calibration curve. For formulation of calibration standards 10 mg/mL BSA stock solution was used.

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Experiment 1-7. Gel Filtration Chromatography: Desalting of a protein containing solution

Results
Gel filtration chromatography In the setoff part of the...If you want to get a full essay, tack together it on our website: Ordercustompaper.com



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